Voyager DE/Calibration

Calibration Procedure

Spot a sample of a the appropriate calibration mix and load an appropriate method
Collect the spectra with the default calibration and open it in Data Explorer
Baseline correct the spectrum (Process > Baseline Correction)
Filter noise from the spectrum (Process > Noise Filter/Smooth)
Manually calibrate the spectrum
- Select Process > Mass Calibration > Manual Calibration
- A window titled Manual Mass Calibration appears, move this window off to the side so you can see all of the peaks of interest
- Select the first peak to calibrate on by using the right mouse button to draw a box around the entire peak
- From the Select or Create Reference Peak Information select the correct mass for the peak you are calibrating and click OK
- Repeat for each calibrant peak
- After identifying all of your peaks click the Plot button in the Manual Mass Calibration window - your peaks will now reflect the new calibration settings
- Click Export to save your calibration settings as a .cal file
Load the calibration file into the Voyager Instrument Control Software
- In the lower right hand corner of the window select External Calibration and browse to the .cal file you created above
Collect your sample spectrum - the calibration settings will be automatically applied

Mass Range: 6,000 to 17,000 Da

Use a 1:10 dilution of 1 mg/ml Cytochrome C and 1 mg/ml Myoglobin in 50 H₂O / 50 ACN + 0.1% TFA
Mix 1:1 with appropriate matrix solution (recommendation: sinapinic acid)

Mass Range: 900 to 1,700 Da

Contains a mixture of Glu1-Fibrinopeptide B, Neurotensin, Angiotensin I, and des-Arg1-Bradykinin

Use full strength
Mix 1:1 with appropriate matrix solution (Recommendation: sinapinic acid - best, or α-cyano-4-hydroxycinnamic acid - OK)

Any combination of the above calibrant mixes, or a solution of any synthetic peptide of known mass can be used to calibrate the maldi.

Calibrant	Charge	Average Mass	Solution to use
Myoglobin	+1	16,952.56	0.1 mg/ml solution in 50/50
	+2	8,8476.78
Cytochrome C	+1	12,361.10	0.1 mg/ml solution in 50/50
	+2	6,181.05
Glu1-Fibrinopeptide B	+1	1571.61	Agilent Cal 1 Mix
Neurotensin	+1	1673.96	Agilent Cal 1 Mix
Angiotensin I	+1	1297.51	Agilent Cal 1 Mix
des-Arg1-Bradykinin	+1	905.05	Agilent Cal 1 Mix

for more information see Section 5 of the Voyager Biospectrometry Workstation: Getting Started Guide Located in the second section (first tab) of the white binder in the drawer under the control computer.